SOLIScript® KIT
SOLIScript® Reverse Transcriptase (RT) is an in-silico designed chimeric RNA‐directed DNA polymerase. SOLIScript® RT can synthesize a complementary DNA strand from ssRNA or ssDNA and is active at higher temperatures (up to 60°C). SOLIScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains an RNase H domain with reduced activity. Applications First-strand cDNA synthesis RT-PCR RT-qPCR
Reagents SOLIScript® Reverse Transcriptase 10x RT Reaction Buffer with DTT, 500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT